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Zymography of type I NAFs (n = 68) revealed four gelatinolytic bands, with molecular masses similar to those obtained for gelatinases from capillary blood, that were biochemically inhibited by EDTA and 1,10-phenanthroline (Fig.
We used the 1,10-phenanthroline method with an ultraviolet-visible spectrophotometer for iron analysis of water samples (Fries and Getrost 1975).
All gelatinolytic bands were confirmed as MMP activity because they were completely inhibited by 5 mmol/L EDTA and 2 mmol/L 1,10-phenanthroline (1).
Both 1,10-phenanthroline and Cu(I)Br are analytical reagent grade and re-crystallization from ethanol before using.
At least 27% of the samples contained an analytical interference to the 1,10-phenanthroline methods for measuring Fe(II) and total Fe.
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