For this purpose, firstly the L citriodora extract was characterized and its radical scavenging activity was measured by 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay.
myeloperoxidase; DPPH, 2,2-diphenyl-1-picrylhydrazyl.
Antioxidant capacity of the both olive leaf extract and quercetin was measured using the DPPH assay based on the scavenging ability to 2,2-diphenyl-1-picrylhydrazyl
(DPPH) stable radical (Goupy et al 1999).
Among these are the methods that use azo-compounds to generate peroxyl radicals, such as the "TRAP" method (Total Radical-Trapping Antioxidant Parameter) and ORAC; the scavenging of radical cation by 2,2-azinobis-(3-ethylbenzothiazoline-6-sulphonate) such as the ABTS or "TEAC" method (Trolox Equivalent Antioxidant Capacity); the scavenging of stable radical by 2,2-diphenyl-1-picrylhydrazyl
known as the DPPH method and the scavenging of radical cation by N,N-dimethyl p-phenylenediamine for the DMPD method.
The antioxidative capability of the compounds under study has been evaluated by using different assays like a ferric reducing/antioxidant power assay (FRAP), an oxygen radical adsorption capacity method (ORAC), colourization assays using stable coloured free radicals, such as 2,2-diphenyl-1-picrylhydrazyl (DPPH), a 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radical cation or a reactive oxygen species, due to their intensive absorbance in the visible region [2, 35-38].
Besides, the antioxidative capability of the compounds in question was determined spectrophotometrically, using a free radical like 2,2-diphenyl-1-picrylhydrazyl (DPPH).
Known methods include the active oxygen method (AOM) and its automated versions (Rancimat and SAFFEST) for lipophilic phases, and 2,2-Diphenyl-1-picrylhydrazyl
(DPPH) or oxygen radical absorption capacity (ORAC) for aqueous environments.
assay gave the highest antioxidant activity for EOOK compared to the oil from O.
All reagents include Folin-Ciocalteu reagent, 2,2-diphenyl-1-picrylhydrazyl
, gallic acid, sodium carbonate, vitamin E, butylated hydroxyanisole, EDTA and all solvents (HPLC grade) were obtained from Fluka (Switzerland).
Galic acid, 2,2-diphenyl-1-picrylhydrazyl
(DPPH) and hydrochloric acid 32% were obtained from Sigma-Aldrich.
Folin-Ciocalteu's phenol reagent, 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid (ABTS) and Iron (II) chloride were purchased from Sigma-Aldrich (Steinheim, Germany), Aluminium (III) chloride and methanol were obtained from Merck (Hohenbrunn, Germany), 2,2-diphenyl-1-picrylhydrazyl (DPPH) and Iron (III) chloride were purchased from Fluka (Buch, Spain, Switzerland, Germany) and ethylenediaminetetraacetic acid (EDTA) and trichloroacetic acid was obtained from Riedel-de Haen (Germany).
Malva sylvestris and Mentha pulegium using several tests: 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid (ABTS), reducing power and metal chelating and it is also targeted to determine the total phenolic, and flavonoid contents of these plants using current methods.