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It usually refers to a process that forms reactive ketones or aldehydes that can be reacted by 2,4-dinitrophenylhydrazine (DNPH) to form hydrazones.
The chemical causing concern is called 2,4-dinitrophenylhydrazine.
Spectrophotometric determination of methyl glyoxal with 2,4-dinitrophenylhydrazine.
Abbreviations CPZ: Chlorpromazine PS: Phosphatidylserine AchE: Acetylcholinesterase RBCs: Red blood cells PB3: Band 3 protein Hb: Hemoglobin GEs: Glycolytic enzymes G6P: Glucose-6-phosphate PPP: Pentose phosphate pathway HOS: High-oxygenation state LOS: Low-oxygenation state DNPH: 2,4-Dinitrophenylhydrazine p-NPP: p-Nitrophenylphosphate Met-Hb: Methemoglobin PMSF: Phenylmethanesulfonylfluoride NBT: Nitroblue tetrazolium PMS: Phenazine methosulphate t-BHT: tert-Butyl-hydroperoxide EDTA: Ethylenediaminetetraacetic acid TBARS: Thiobarbituric acid reactive substances OV: Orthovanadate DNP: Dinitrophenyl FACS: Fluorescence-activated cell sorting SD: Standard deviation pHi: Cytoplasmic pH.
In general, passive air samplers for carbonyl compounds, as aldehydes, contain and are based on the reaction of carbonylic compounds with 2,4-dinitrophenylhydrazine (2,4-DNPH).
Although the previous study had proved that eleven hydrazine derivatives, aromatic amines showed direct mutagenicity and toxicity toward Salmonella Typhimurium and associated mutagenic process such derivatives as - phenylhydrazine, 2-nitro-phenyl-hydrazine, 4-nitrophenylhydrazine, 2,4-dinitrophenylhydrazine, p-tolihydrazine, and 4-nitroaniline [16].
Simultaneous removal of heavy-metal ions in wastewater samples using nano-alumina modified with 2,4-dinitrophenylhydrazine, J.
Determination of carbonyl compounds generated from the electronic cigarette using coupled silica cartridges impregnated with hydroquinone and 2,4-dinitrophenylhydrazine.
The most widely used reagent is 2,4-dinitrophenylhydrazine (DNPH) which reacts with aldehydes to form hydrazones which are then determined by HPLC.
Sodium hydroxide, acetic acid, methanol, 2,4-dinitrophenylhydrazine (Brady's reagent, DNP), potassium iodide, ethyl acetate, hydrochloric acid, acetic anhydride, glacial acetic acid, perchloric acid, potassium hydrogen phthalate and Oracet blue indicator, Dragendorff's reagent and vanillin/sulphuric acid reagent were obtained from the Chemical Store of the Departments of Pharmaceutics and Pharmaceutical Chemistry, Faculty of Pharmacy and Pharmaceutical Sciences, KNUST, Ghana.
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