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2-AP2-Aminopurine (molecular marker)
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The s base is an analog of the well-known fluorescent 2-aminopurine base, and the ribonucleoside of s exhibits fluorescence emission centered at 434 nm by excitation at 352 nm in phosphate buffer (pH 7).
Mutant libraries were generated using ethylmethanesulphonate (EMS), 2-aminopurine, and ultraviolet light.
The addition of 5-methylcytosine and 2-aminopurine adjacent to the deletion improved discrimination dramatically.