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AAG |
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Financially troubled AAG is reducing its long-haul fleet by
four A330-200s and two A340-300s. In step
1, PCR of DNA on the disks or from elutant was amplified with primers
rPLU1 (5'-TCA AAG ATT AAG CCA TGC AAG TGA-3') and rPLU5
(5'-CCT GTT GTT GCC TTA AAC TCC-3') in a standard 50-[micro]L
PCR with a PTC-200 Thermocycler (MJ Research; Waltham, MA, USA) under
the conditions of 94[degrees]C for 4 min, and 35 cycles at 94[degrees]C
(30 see), 55[degrees]C (1 min), 72[degrees]C (1 min), with an additional
extension at 72[degrees]C (4 min). The AAG will be retrofitted into existing Nimitz-class
aircraft carriers and installed in follow-on types as they are built. |
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