Grape and grape seed extract capacities at protecting LDL against oxidation generated by Cu2+, AAPH
or SIN-1 and at decreasing superoxide THP-1 cell production.
2] protocol, 10 healthy participants (4 males; mean age, 32 years; range, 23-51 years) were recruited; 10 additional participants (3 males; mean age, 30 years; range, 21-49 years) were recruited to the AAPH protocol.
001) and a modest, nonsignificant decrease when AAPH was the initiating oxidant (Fig.
o conde da Ericeira, 1721-22', AAPH
, 2nd series, 15 (1965), 243-50.
After a washing step, AAPH
dissolved in HBSS is applied at a final concentration of 600 [mu]M to the cells, and they are incubated for 45 min at 37[degrees]C.
Plasma samples from the volunteers were assessed for lipid peroxidation by exposure to 50 mmol/L AAPH
The method of Glazer (5) assumes that the decrease in fluorescence of B-phycoerythrin (B-PE) or R-phycoerythrin (R-PE) in the presence of 2,2'-azobis(2-amidinopropane) dihydrochloride (AAPH
) is linear with time and that a period of complete protection (the length of the lag phase) of B- or R-PE against AAPH
is related to antioxidant concentrations.
2+] and AAPH
when the three oxidants were at the concentrations indicated in the Table.
Briefly, 1 mL of RBC suspensions was mixed with equal volumes of NaCl (9 g/L) containing AAPH
3) FY09 underlying earnings adjusted for pounds-106m one-off gain related to internal restructuring of an annuity portfolio, pounds+16m dividend on AAPH
shares and on the business retained
5] cell/ml overnight, followed by the addition of DFE, 1 [micro]M DCFH-DA and 1 mM AAPH
The addition of AAPH
(a peroxyl radical initiator) to the suspension of RBC causes oxidation of lipids and proteins in cell membrane and thereby induces hemolysis.