tenella leaves based on DPPH, ABTS
and FRAP assays (Table 3, 4, 5).
Figure 2 shows the results of the antioxidant capacity for the jam formulations using the ABTS
A novel automated direct measurement method for total antioxidant capacity using a new generation, more stable ABTS
These studies commonly use FRAP, DPPPH, ABTS
and ORAC methods in which free radicals and their formation are inhibited.
The hydroxyl radicals scavenging activity was calculated according to the equation given under ABTS
Aehr Test has developed and introduced several innovative products, including the ABTS
, FOX(TM), MTX and MAX systems and the DiePak(R) carrier.
b) Antioxidant capacity level was measured using the ABTS
Folin-Ciocalteu reagent in sodium carbonate, gallic acid, catechin, ABTS
(2,2 '-azino-bis (3ethylbenzthiazoline-6- sulfonic acid) were purchased from Fluka (Germany).
Antioxidant capacities of the prepared tea samples were determined by measuring the amount of reduced ABTS
radical monoca-tion (2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid)).
is an excellent substrate for peroxidases and is frequently used to study for antioxidant properties of natural compounds and electron transfer reactions involving free radicals (Reszka, 2007).
is based on the suppression of the absorbance of radical cations of 2,2'-azinobis(3-ethylbenzothiazoline 6-sulfonate) (ABTS
) by antioxidants in the test sample when ABTS
incubates with a peroxidase (metmyoglobin) and [H.
Among these are the methods that use azo-compounds to generate peroxyl radicals, such as the "TRAP" method (Total Radical-Trapping Antioxidant Parameter) and ORAC; the scavenging of radical cation by 2,2-azinobis-(3-ethylbenzothiazoline-6-sulphonate) such as the ABTS
or "TEAC" method (Trolox Equivalent Antioxidant Capacity); the scavenging of stable radical by 2,2-diphenyl-1-picrylhydrazyl known as the DPPH method and the scavenging of radical cation by N,N-dimethyl p-phenylenediamine for the DMPD method.