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To validate the assay, 2 skim breast milk samples with the baseline AFABP concentrations of 8.
No crossreactivity was found with recombinant human AFABP (Biovendor), IFABP (R&D Systems), LFABP (Abnova), or other related proteins: leptin, leptin receptor, adiponectin, resistin, and RELM-(3 at 100 [micro]g/L and IL-6 at 2 [micro]g/L (all provided by Biovendor).
There was no relationship with the number of previous pregnancies or deliveries or any other variable in mothers or newborns, nor with AFABP, EFABP, or leptin milk concentrations.
Age- and sex-adjusted serum concentrations of AFABP were inversely associated with circulating concentrations of adiponectin, an important adipokine with antidiabetic and antiatherogenic activities (32).
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