4] Nonstandard abbreviations: RT, reverse transcripfion; PS, polystyrene; AGCT
, Advanced Gene Computing Technologies; PP, polypropylene; G3PDH, glyceraldehyde-3-phosphate dehydrogenase; and DEPC, diethylpyrocarbonate.
Recently, a single, somatic mutation of the gene FOXL2 402[right arrow]CrG (C134W) was reported in virtually all aGCTs in women.
In this study, polymerase chain reaction (PCR) and direct sequencing were performed in 5 aGCTs from male patients to investigate whether FOXL2 402[right arrow]CrG (C134W) also occurs in granulosa cell tumors arising in men.
Five cases of aGCTs arising in males were selected from the consultations files of the Division of Anatomic Pathology at the Mayo Clinic (Rochester, Minnesota).
All cases had classic histopathologic features of aGCTs and are illustrated in Figure 1, A through E.
FOXL2 402[right arrow]CrG (C134W) was identified in 40% (2 of 5) of the aGCTs (cases 1 and 3).
Recently, ovarian aGCTs were shown to have a point mutation in FOXL2.