AHDFAmerican Horse Defense Fund
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AHDF surveyed a number of rendering plants, vets and crematoriums and the costs of euthanasia and disposal are listed in our book.
AHDF recently rescued a mustang that was sent to auction and would have been sold to slaughter.
After incubation for 1, 3 and 5 ci, cell numbers were determined by MTT assay for aHDFs as described above, or a 5-bromo-2'-deoxyuridine (BrdU) incorporation assay (Roche Applied Science, Basel, Switzerland) for aNHEKs.
In the cell viability assay, asiaticoside was not cytotoxic in aHDFs at any concentration from 61.
2, (A) and (C) show the numbers of aHDFs and aNHEKs attached as assessed by MIT assays, and (B) and (D) show the cell attachment areas of aHDFs and aNHEKs, respectively, as investigated by fluorescent staining.