In the present study, we studied the anti-heat stress effects of AHSP through biochemical measurements and microscopic observations, and evaluated the toxicological safety of AHSP.
Heat stress and AHSP Animals: Mice were purchased from the Laboratory Animal Center, Sun Yet-Sen University of Medical Sciences.
Drugs and agents: AHSP, developed by the Key Laboratory of Tropical Medicinal Plant Chemistry of Ministry of Education, was confected with honeysuckle, astragalus, ophiopogon, schisandra, diffusa, bupleurum, and g-aminobutyric acid (GABA) according to a certain proportion (Chen et al.
Animal groups and sample collecting: After 3 days adaptive feeding, mice were randomly divided into four groups: the control group, the AHSP group, the HS group, and the (AHSP+HS) group.
For the control group and the AHSP group, blood was collected without prior heat stress stimulation.
AHSP acute toxicity test: The AHSP acute toxicity test was performed as described previously (Xu, 2001).
In contrast, AHSP administration reduced the activities of LDH and CK and the concentration of LD in the plasma of heat stress treated mice, which, however, were still higher than in the control group and the AHSP group.
In the meanwhile, the AHSP administration can significantly increase the SOD activity (p(less then)0.
1, Under a microscope, we found that compared to the control group and the AHSP group, heat stress caused a series of changes in the structure of the livers and intestines.
Diamant]-globin chains in the AHSP association constant could explain the observed differences in the clinical and biochemical phenotypes of these 2 Hbs (7).
Impaired binding of AHSP to [alpha] chain variants: Hb Groene Hart illustrates a mechanism leading to unstable hemoglobins with [alpha] thalassemic like syndrome.
6] Nonstandard abbreviations: Hb, hemoglobin; AHSP, [alpha]-Hb stabilizing protein; RPLC, Reversed-phase liquid chromatography; LC, liquid chromatography; ESI MS, electrospray ionization mass spectrometry; MALDI-TOF, matrix-assisted laser desorption/ionization time-of-flight; [[alpha].