The purification of rPfLDH was done by IMAC affinity chromatography using AKTA
One example of disposable technology is the AKTA
ready Liquid Chromatography system designed for process scale-up and production for Phase I-III drug development and full scale production.
Amersham Biosciences, which makes the AKTA
prime, remains the leader in the field with approximately 25% of the market and a commanding presence in prep LC.
member was allowed to choose one student per studio to perform for this worthy cause.
Development of purification method on the of anion exchange chromatography was carried out using a 5 mL DEAE Sepharose anion exchange column (Toyo, Japan) in an AKTA
explorer system (GE Healthcare, USA) as per the instructions given by the manufacturer.
The results of the present study do not constitute endorsement of a product by the AKTA
The purification was conducted using AKTA
purifier[TM] (GE Healthcare Bio-Sciences, Sweden).
02 mg pepsin and 100 [micro]l protein, purified by the AKTA
system, were diluted into 900[micro]l activation buffer, consisting of 200 mM Na phosphate, 5 mM EDTA, 5mMDTT, pH 4.
We used an AKTA
fast-performance liquid chromatography system (FPLC) running Unicorn v4.
Centrifuge (Sorvall Evolution RC, USA), ultrasonic disintegrator VibraCell VCX 750 (Sonics, USA), ELISA reader (Bio-Tek, USA), electrophoresis apparatus Mini-Protean III and Semi Dry blotting system (Bio-Rad, USA), Shaker with incubator (ISF-1-W, Kuhner, Switzerland), Spectrophotometer (Biomate 3, Thermo Electron Corporation, U SA), AKTA
explorer (GE Healthcare, Sweden) were used in the present study.
The column was attached to the device AKTA
prime and equilibrated with 60 to 90 ml of sodium phosphate buffer 1x at a flow rate of 1 ml/min.