ALCAM

AcronymDefinition
ALCAMActivated Leukocyte Cell Adhesion Molecule
ALCAMAtlas Linguistique du Cameroun (French)
References in periodicals archive ?
Expression and regulation of ALCAM mRNA in the uterine endometrium during the estrous cycle and pregnancy in pigs
To determine ALCAM mRNA levels in the uterine endometrium during the estrous cycle and pregnancy in pigs, we conducted real-time RT-PCR analysis.
Localization of ALCAM mRNA in the uterine endometrium during the estrous cycle and pregnancy
To determine which cell types express ALCAM mRNA in the uterine endometrium during the estrous cycle and pregnancy and in conceptuses, we performed in situ hybridization analysis using uterine endometrial tissues from D12 and D15 of the estrous cycle and pregnancy and conceptuses from D12 of pregnancy.
Expression of ALCAM in the conceptus during early pregnancy
To determine whether conceptuses express ALCAM mRNA during the implantation period, we analyzed ALCAM expression in conceptuses from D12 and D15 of pregnancy by RT-PCR and found ALCAM mRNA expression on both D12 and D15 of pregnancy (Figure 3).
Because ALCAM expression was highest on D12 of pregnancy in the uterine endometrium, we hypothesized that ALCAM expression might be regulated by estrogen secreted by elongating conceptuses and progesterone of ovarian origin.
Comparison of ALCAM expression levels in the uterine endometrium in pigs carrying Non-NT and NT-derived embryos
To determine influence of ALCAM expression on the establishment of pregnancy in recipients with SCNT embryos, we compared ALCAM mRNA expression levels in the uterine endometrium of pigs carrying Non-NT and SCNT-derived embryos by real-time RT-PCR.
In the present study, we determined in pigs that i) ALCAM expression in the uterine endometrium changed during the estrous cycle and pregnancy with the highest levels on D12 of pregnancy; ii) ALCAM mRNA was localized in the luminal and glandular epithelial cells in the endometrium; iii) ALCAM expression was detected in conceptuses on D12 and D15 of pregnancy, localized to the conceptus trophectoderm; iv) steroid hormones (estrogen and progesterone) did not regulate ALCAM expression in the uterine endometrium; and v) ALCAM expression levels in the uterine endometrium were not different between pigs carrying SCNT and Non-NT-derived embryos.
We observed that ALCAM expression was differentially regulated in the uterine endometrium during pregnancy in pigs.
ALCAM was localized to the luminal and glandular epithelial cells and the trophectoderm of conceptuses during early pregnancy.