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AP-PCR

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AcronymDefinition
AP-PCRArbitrarily Primed Polymerase Chain Reaction Technique


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RAPD RAPD (Random Amplified Polymorphic DNA) ou AP-PCR (Arbitrarily Primed PCR), foram descritas, originalmente, por Welsh e McClelland em 1990, e envolvem o uso de um primer unico e pequeno (usualmente de 10 a 15 bases), arbitrariamente escolhido, para amplificar DNA genomico em condicoes de baixa estringencia, nao sendo necessario o conhecimento previo da regiao de ligacao do primer (Welsh e McClelland, 1990; Williams et al.
Amplifications of the different markers were performed as previously described: tdh and trh (7), orf8 (11), toxRS/new (4), and AP-PCR (11).
Bacterial DNA for AP-PCR was obtained, and amplification was performed (5).
 
 
 
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