APAAPAlkaline Phosphatase-Anti Alkaline Phosphatase
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Western blot and APAAP were performed with the same antibodies as for flow cytometry.
For each marker, Western blot and APAAP results confirmed the expected positivity and negativity of the control CLs.
Positive prostate cells were visualized by the alkaline phosphatase anti-alkaline phosphatase staining procedure, following manufacturer's recommendations (Dako APAAP kit[R]).