APLP2Amyloid Precursor-Like Protein 2
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The researchers believe that increased levels of the APLP2 protein in the eye cause it to lengthen, resulting in myopia.
Membranes were prehybridized in Rapid-Hyb buffer (Amersham) at 65 [degrees] C for 30 rain and hybridized for 4 hr at 65 [degrees] C with APP, APLP2, [beta]-actin, or S7 cDNA probes labeled with [[alpha]32]dCTP using a random priming kit (Amersham).
5% Tween 20) for 1 hr, and then incubated with anti-mouse APLP2 antibody D21 1 (31) 1:2,500 dilution in 5% skim milk, TBS-T) or anti-mouse neuron-specific [beta]-tubulin monoclonal antibody Tuj1 (32) (1:25 dilution in 5% skim milk, TBS-T) at room temperature for 3 hr.