One possibility is that that the virus entered Georgia through meat products since ASFV may remain viable for long periods in infected pig tissues, meat, and processed pig products.
Dr Rowlands studied for her PhD at the Institute for Animal Health Pirbright Laboratory on the interaction of ASFV with the tick vector Ornithodoros erraticus and on the molecular epidemiology of ASFV.
Determination of the sequence of the ASFV
isolate that was introduced into the Caucasus region provides a benchmark to which other isolates from this epidemic can be compared.
In June 2007, ASFV
was identified in the Caucasus region, including Georgia, Russian Federation, and Armenia (2).
Collected ticks were tested for ASFV
infection by nested PCR amplification of the VP72 gene, a method considered most sensitive for detection of viral DNA in ticks (14).
Researchers at the National Research Institute for Veterinary Virology and Microbiology (VNIIVViM) in Pokrov, Russia, have developed a classification of ASFV
isolates based on a hemadsorption inhibition assay (HAI) with ASFV
reference immune antisera.
After the presence of ASFV
was confirmed in samples, initial genetic characterization was performed by using standardized genotyping procedures on virus DNA extracted directly from homogenized tissues and from bone marrow samples.
Historically, all ASFV
p72 genotypes have been circulating in eastern and southern Africa, and genotype I has been circulating in Europe, South America, the Caribbean, and western Africa (2,3).
Most field strains of ASFV
can persistently infect Ornithodoros ticks, including the species O.
6] 50% tissue culture infectious dose of the ASFV
The aim was to define clinical signs, disease dynamics, and postmortem lesions in wild boars after intramuscular and oral infection with ASFV
Clinical material was sent to the European Union Reference Laboratory for African Swine Fever (Centro de Investigacion en Sanidad Animal, Madrid, Spain) for confirmatory diagnosis and characterization of the ASFV
strain(s) responsible for the outbreak(s).