Dynamic changes in microbial amylase and hemicellulase activities in ATMR and LTMR silages during ensiling are shown in Figure 1.
A total of 62 and 80 enzyme-producing strains were isolated from ATMR and LTMR silages, respectively.
The effects of pH on the amylase and hemicellulase activities of microbes isolated from ATMR and LTMR silages during ensiling are shown in Table 6.
In this study, although the initial pH and lactic acid concentration were different between ATMR and LTMR, which preumably caused by the different properties between alfalfa hay and Leymus chinensis hay, both TMR silages obtained good fermentation quality after 56 days of ensiling as indicated by the high concentration of lactic acid, low pH and N[H.
In this study, eight species that produced amylase or hemicellulase were isolated from ATMR, LTMR, and their silages.
In ATMR and its silages, five amylolytic species, B.
After 28 d of ensiling, the time of increase in temperature extended to 120 and 72 h, and aerobic deterioration was observed at 148 and 90 h for ATMR silage and LTMR silage, respectively.
A different pattern was observed in TMR silages: the LAB populations showed a slight downward trend during the first 120 h in ATMR silage and 72 h in LTMR silage, regardless of ensiling time, and then increased gradually until they were stable.
In 28-d and 56-d ATMR silages, pH remained stable until 120 and 216 h, respectively.
For both TMR, the lactic acid concentrations initially increased and then declined during air exposure, peak concentrations were observed at 24 h in ATMR and 12 h in LTMR.
acidilactici lost their dominant position in the later stage of aerobic deterioration in ATMR and LTMR, respectively, P.
05) in 56-d ATMR silage than in 28-d ATMR silage, while no significant differences (p > 0.