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| Acronym | Definition |
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| AFL | American Federation of Labor | | AFL | American Football League (1960s) | | AFL | Australian Football League | | AFL | Arena Football League | | AFL | Alberta Federation of Labour | | AFL | Afloat | | AFL | Aeroflot (Russian Airline, ICAO code) | | AFL | Armed Forces of Liberia | | AFL | Atrial Flutter | | AFL | Alcoholic Fatty Liver | | AFL | Abstract Family of Languages | | AFL | Above Floor Level | | AFL | Artists for Literacy (San Francisco, CA) | | AFL | After Fader Listening (audio mixing) | | AFL | Autofocus Lock (cameras) | | AFL | Air Force Letter | | AFL | AbsoFreakinLutely | | AFL | Authorized Funding Level | | AFL | Airborne Flying Laboratory | | AFL | Active Fuel Length | | AFL | Autoland Flight Tests | | AFL | Approximate Full Load | | AFL | Applied Fisheries Laboratory | | AFL | Automatic Line Feed | | AFL | Away From Link | | AFL | Auxiliary Fuel Line | | AFL | Adaptive Forward Lighting (automotive engineering) | | AFL | Association Française du Lupus (French: French Lupus Association) | | AFL | Active for Life (various organizations) | | AFL | Association Française de Limnologie (French: French Limnology Association) | | AFL | Association Familiale du Limousin (French: Limousin Family Association; Limousin, France) | | AFL | Assessment for Learning (education) | | AFL | Association Française pour la Lecture (French: French Reading Association) | | AFL | Acemai France Logique (French elevator manufacturer) | | AFL | Alcoholics for Life |
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