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APCAmerican Power Conversion
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References in periodicals archive ?
Key words: allophycocyanin (APC); equivalent reference fluorophores (ERF); fluorescein isothiocyanate (FITC); fluorescence; multicolor flow cytometer; Pacific Blue (PB); phycoerythrin (PE).
This manuscript describes a procedure to establish a linear scale in four fluorescence channels (fluorescein isothiocyanate (FITC), phycoerythrin (PE), allophycocyanin (APC), and Pacific Blue (PB)) of a multicolor flow cytometer.
Reference Fluorimeter Microsphere fluorophore Setting calibrator [surface labeled, 2-4 [micro]m) Fluorescein (SRM) Ex: 435 nm Em Fluorescein 495-700 nm isothiocyanate (FITS) Nile Red Ex: 488 nm Em Phycoerythrin (RE) 495-700 nm Allophycocyanin ( Ex: 533 nm Em ARC APC) 540-700 nm Coumarin 30 Ex: 405 nm Em: Pacific Blue (PB) 410-1300 nm
Cole at the Biochemical Science Division of the NIST Material Measurement Laboratory for his help in the determination of the concentration of the allophycocyanin stock solution by amino acid analysis.
Phycofluors primarily Phycoerytherin and Allophycocyanin are used in fluorescence-activated cell sorting, flow cytometric analysis and histochemistry.
Phycobiliproteins were extracted by subjecting the cells to mild sonication in phosphate buffer and repeated freezing and thawing; the amounts of Phycocyanin (PC), Allophycocyanin (AC) and Phycoerythrin (PE), absorption maxima of the supernatant were determined spectrometrically and a quantitative estimation was done according to Bennett and Bogorad [7].
iyengarii cells increased upon illumination of the cells in Phycocyanin and Allophycocyanin absorption region compared to that of low and high light adapted cells.
Antibodies were conjugated with fluorescein isothiocyanate, phycoerythrin, peridinin chlorophyll protein, or allophycocyanin.
A flow cytometer FACSCalibur (BD Biosciences) was used, and CaliBRITE 3 and CaliBRITE allophycocyanin beads (BD Biosciences) were used to validate the instrument performance.
Antibodies conjugated commercially to fluorescein isothiocyanate (FITC), phycoerythrin, peridinin-chlorophyllprotein (PerCP), and allophycocyanin (APC) dyes were used.
The following antibodies were used: anti-TCR[alpha][beta] FITC, anti-CD4 FITC, anti-CD69 phycoerythrin (PE), anti-CD3 peridinin chlorophyll-[alpha] protein (PerCp), and anti-CD4 allophycocyanin (Becton Dickinson).
The PBXL-1 dye, used in the thyroidstimulating hormone (TSH) model, contains B-phycoerythrin, R-phycocyanin, and allophycocyanin as its component phycobiliproteins.