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The new system, which consists of a lab-on-a-chip, laser-induced fluorescence detection device and analysis method based on antigen-antibody reaction and electrophoresis, can rapidly measure stress-related substances secretory immunoglobulin A (S-IgA) and cortisol in salivary samples. The antigen-antibody complexes were visualized using the chemoluminescence substrate SuperSignal West Pico (Pierce, Rockford, IL) as recommended by the manufacturer. It has been postulated that they might be the result of a postinflammatory repair process, a metabolic disturbance, or an antigen-antibody interaction with an agent that was no longer identifiable in aspiration or biopsy material. |
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