| Acronym | Definition |
|---|
| ANI | Automatic Number Identification |
| ANI | Animation (file extension) |
| ANI | Accord National Interprofessionnel (French: National Interprofessional Agreement) |
| ANI | Autism Network International |
| ANI | All Natural Ingredients (band) |
| ANI | Automatic Number Identifier |
| ANI | Association Nationale des Iconographes (French: National Association of Iconographers) |
| ANI | Aniak, AK, USA (Airport Code) |
| ANI | African Networking Initiative (now Partnership for Information and Communication Technologies in Africa) |
| ANI | Afrique et Nouvelles Interdépendances (French: Africa News and Interdependencies) |
| ANI | Administrators' Noticeboard/Incidents (Wikimedia Foundation) |
| ANI | Authorized Nuclear Inspector |
| ANI | Animated Windows Cursor (Microsoft filename extension) |
| ANI | Acute Nerve Irritation |
| ANI | Actual Net Interchange |
| ANI | Automated Name Index (California DMV vehicle registration lookup) |
| ANI | American Nuclear Industries |
| ANI | Assistant NATOPS Instructor (USMC) |
| ANI | Anjuman Naujawanan-i-Islam (Pakistan) |
| ANI | Association Nationale Ibn-Al-Awam |
| ANI | Airborne Network Integration |
| ANI | Atmosphere Normale Internationale |
| ANI | Agence de Nettoyage Industriel (French: Industrial Cleaning Agency) |
| ANI | Avex Network, Inc. (Japan) |
| ANI | Asian News International |
| ANI | Advanced Network Infrastructure |
| ANI | Armenian National Institute |
| ANI | Audubon Nature Institute (New Orleans, LA) |
| ANI | Andaman and Nicobar Islands |
| ANI | Access Network Interface |
| ANI | Atkins Nutritionals, Inc. (Denver, CO) |
| ANI | American Nuclear Insurers (Glastonbury, CT) |
| ANI | Am Not Interested |