Kallikrein is known to possess arginine ester hydrolase activity and thus is able to cleave the ethyl ester group from the synthetic substrate BAEE ([alpha]-N-benzoyl-L-arginine ethyl ester).
Fractions containing 59% of the total BAEE hydrolase activity from the second G-75 column were pooled and rechromatographed on a third G-75 column (50 mM Tris-HCl, 50 mM KCl, pH 7.
Fractions containing 92% of the total BAEE hydrolase activity were applied to a DEAE Sephadex A-50 ion exchange column (2.
By the standard of BAEE exhibitions during the 1980s, the number of new products was smaller than normal.
Several technology demonstration units operating in different frequency bands have now been built and tested, and the unit on display was delivered to the Royal Signals and Radar Establishment (RSRE) several years ago, but had been borrowed back to make its public debut at BAEE.
The inhibitory activities on bovine pancreatic trypsin and bovine-chymotrypsin were determined by measuring the remaining hydrolytic activity toward specific substrates, BAEE
and BTEE, respectively, after pre-incubation with PDTI for 10 min.