BCECF2',7'-Bis- (2-Carboxyethyl)-5- (And-6)- carboxyfluorescein (molecular probes)
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The fluorescence intensity of BCECF is dependent upon the pH with a maximum response at an excitation of [lambda]=535 nm (F1), while at [lambda]=490 nm (F2), the intensity is independent of pH.
The total fluorescence intensity of BCECF labelled YAC1 cells (Target-T) was determined after adding 1.
In this context, it is interesting to note that glutamate has previously been reported to promote an acidification of the internal milieu of catfish retinal horizontal cells as measured using the pH-indicator dye BCECF [11].