pneumophila isolates were cultured on BCYE
agar, and their growth was confirmed using a serotyping kit.
bethesdensis after 7 days of incubation on BCYE and mycobacterial media.
bethesdensis within 3 weeks on a variety of media (Middlebrook 7H11, BCYE, and fungal media), but growth was sparse.
45 [micro]m membrane filters, the filter was placed on BCYE agar, and incubated in a candle jar at 37 [degrees]C for 72 h.
DNA was extracted from samples, following 72 h incubation on BCYE plates, using InstaGene Matrix (BioRad; Hercules, California USA).
The swab was then lightly streaked directly onto BCYE and then immersed in 2.
Catalase positive and oxidase negative colonies showing the typical morphology were subcultured on plain BCYE and on blood agar (BA) to determine if L-cysteine is essential for growth (13).
This organism will not grow on Gram-negative media but will grow on BCYE
pneumophila (ATCC 331522) is maintained on BCYE
agar and used as a control for culture of clinical specimens.
When Gimenez-stained bacteria were observed, the culture was subcultured on BCYE
agar (BioMerieux, Marcy L'Etoile, France) and sheep blood agar (BioMerieux) and on amoebal microplates without antibiotics or with vancomycin (10 [micro]g/mL) or colistin (500 U/mL).
The procedure for isolating bacteria from water and lung secretions by using cocultivation with Acanthamoeba polyphaga followed by subculture onto BCYE
agar plates has been detailed elsewhere (11,26).
pneumophila has been isolated by using HEL cells while inoculated BCYE
and agar plates remained sterile (47).