To study the cell adhesion behaviour of the BGHA block, osteoblast cells (Human osteoblast cell line--HOS) were seeded on the surface of conditioned BGHA block (pre-incubated for 24 h in the medium) and maintained in Minimal Essential Medium supplemented with 10 % fetal bovine serum and 100 units/ml of penicillin and 100 [micro]g/ml streptomycin, respectively and incubated at 37 [degrees]C in humid atmosphere and 5 % C[O.
The cells seeded BGHA block was fixed in 3 % gluteraldehyde in phosphate buffer (pH 7.
The cells seeded BGHA block was fixed in 10 % neutral buffered formalin, rinsed with PBS, and dehydrated in graded acetone series.
SEM micrograph of BGHA block revealed the roughness and the pores of the material.
Under TEM at higher magnification, submicron crystallites (200-400 nm) of BGHA were observed.
BGHA did not show any signs of toxicity after 48 h with L929 cells (Fig 6).
Test on extract (Based on ISO 10993-5, 1999): Extracts of porous HA and BGHA, were prepared by incubating the materials in saline at 37[degrees]C for 24 h at an extraction ratio of 0.
BGHA granules (test material) were implanted in distal and proximal critical size defects in the right femur of the animal.
a) depicted BGHA and HA granules to be of size 300 to 500 [micro]m with well-defined pores of approximately 100 [micro]m with interconnections which is ideal for osteoblast invasion and proliferation.
The EDAX spectrum of BGHA showed silica peaks apart from Ca and P peak and the elemental composition in the material is Si = 6.
The properties of HA and BGHA differ with respect to the preparation route and reaction ingredients.
At a higher magnification using TEM, submicron crystallites of HA and BGHA were seen confirming the materials crystallinity with respect to their unit crystal arrangement (hexagonal) with characteristic diffraction spots.