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Simplified plaque reduction neutralization assay for dengue viruses by semimicro methods in BHK-21 cells: comparison of the BHK suspension test with standard plaque reduction neutralization. 1996 BHK cells Arsenite 3T3-L1 adipocytes McDowell et al. Viral RNA was extracted from the first BHK cell passage by using Trizol (Gibco BRL, Bethesda, MD), according to the manufacturer's protocol, and subjected to reverse transcription with a primer of sequence T25V designed to anneal to the poly (25) tract of the genomic and subgenomic RNA. |
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