Although Herrod had been allotted two British field guns to support his advance, the commander of the guns could not be found at BHQ
By incorporating molecular beacons with Biosearch fluorophores and BHQ
dyes, Micronics' test cartridges can perform either single or multiplex pathogen detection.
Weyfringe Barcode & Labelling Systems, Longbeck Road, Marske, Redcar, Cleveland TS11 BHQ
FAM and BHQ
are the fluorophore and quencher pair used.
3] Nonstandard abbreviations: gPCR, quantitative real-time PCR; Tm, melting temperature; FAM, 6-carboxyfluorescein; TAMRA, 6-carboxytetramethylrhodamine; BHQ
, Black Hole Quencher-2; FRET, fluorescence resonance energy transfer; GcTm, Gaussian curve fit analysis of Tm.
b) F, forward PCR primer; R, reverse PCR primer; RT, reverse transcription primer; FAM, 5,6-carboxyfluorescein; BHQ
, Black Hole Quencher; TXR, Texas Red.
Probes were synthesized with a 5' 6-carboxy-fluorescein reporter and an internal quencher (either BHQ
1 [type A] or QSY-7 [type B]) at the nucleotide position indicated by the quotation marks.
Primers were labeled at the 5' and 3' ends with Texas Red and BHQ