BMMCs were washed twice with PBS and stained with FITC-labeled CD117 and PElabeled IgE receptor for 20 min under room temperature.
Figure 2(a) illustrates the morphology of BMMCs containing an abundance of purple granules after toluidine blue staining.
The data were consistent with the description of exosomes based on size and morphology, suggesting that BMMCs constitutively released exosomes.
a) Western blot revealed the expression of OX40L in BMMCs and their exosomes, but a lower expression on exosomes; (b) flow cytometry showed that naive [CD4.
The levels of IL-5, IL-13, TNF and IFN-[gammma] were determined in the supernatants of BMMC culture or in the BAL fluid by ELISA using recombinant murine cytokines and antibodies according to the manufacturer's instructions (BD Pharmingen, Heidelberg, Germany).
mast cells were low in BMMCs treated with QE, QI or Kp (<10%, <-10%, <-11%, respectively) compared to BMMCs treated with PBS (<-10%).
Similarly to the effects on BMMCs observed in vitro, sensitized and challenged mice treated with Kp or QE showed a significant reduction of airway reactivity to MCh.
A separate study, carried out by researchers in the Peoples' Republic of China, found that apelin, a newly described inotropic peptide, improves heart function following transplantation of BMMCs
To examine the interaction between exposure to environmental estrogens and allergens in the release of allergic mediators, we sensitized BMMC for 1 hr with 100 ng/mL mouse anti-dinitrophenyl (DNP) IgE antibody (Sigma-Aldrich) and HMC-1 cells for 90 min with a 1: 5 dilution of patient serum.
Environmental estrogens enhance IgE-mediated degranulation of HMC-1 cells and BMMC.
2] did not induce significant degranulation from BMMC derived from ER-[alpha] KO mice (Zaitsu et al.
This proposition was substantiated by demonstrating that BMMCs derived from ER-[alpha] knockout (KO) mice did not degranulate in response to [E.