According to the experimental purpose, BOEC pellets were suspended and cultured in DMEM containing 10% FBS for 2-3 days on 4-well plates (a density of 1x[10.
Repeated measures ANOVA was used to test the main effects of melatonin and SNP on cell viability and LPO, and subsequent development rates of bovine IVM/IVF embryo with BOEC co-culture.
The cell viability of BOEC in 50 [micro]M and 100 [micro]M SNP groups increased significantly compared with 1,000 [micro]M and 2,000 [micro]M SNP groups (p<0.
The cell viability, LPO and expression of apoptosis and antioxidant genes in BOEC pre-treated with melatonin (1,000 [micro]M) in the presence or absence of SNP (1,000 [micro]M) are indicated in Figure 3.
facilitators lead post-activity discussions about what worked and what didn't.
BOEC utilizes a 39-acre program site and base camp in Breckenridge at an altitude of 9,600 feet.
The BOEC outdoor curriculum draws on tailor-made programs of varying length.
BOEC is a nonprofit corporation governed by a 15-member and board of directors.
The present study was designed to determine 1) the effects of the BOEC co-culture system on in vitro development rate of bovine IVM/IVF embryos, 2) the effect of status of BOEC on in vitro development of bovine embryos, and 3) detection of the temporal patterns of expression of growth factor genes (TGF, EGF and IGFBP), antioxidant genes (Cu/Zn-SOD, Mn-SOD, Catalase and GPx) and apoptosis genes (Bax, p53 and Caspase-3) in different types of BOEC co-cultured with or without bovine IVM/IVF embryos.
BOEC monolayer was dissolved with Trypsin-EDTA (Gibco).
Following the different cell status, the morphology of BOEC was evaluated by visual examination under phase-contrast microscopy.
Total RNA was also extracted by the guanidium isothiocyanate method (Chomczynski and Sacchi, 1987) from different status of BOEC or from BOEC monolayer which had been used or not used for embryo culture.