Although B[a]P was metabolized to BPDE
in HCC cells, to what extent BPDE
mediates the effects of B[a]P on HCC progression remains unknown.
It has been hypothesized that BPDE preferentially targets methylated CpG sites.
An alternative hypothesis suggests that binding of BPDE to DNA encourages methylation of DNA by influencing DNA methyltransferase activity (Subach et al.
Pipeline volumes expressed in terms of BPDE
are on an energy equivalent basis where 3.
These data derive from control cells and from cells harvested 24 hr after being treated 4 hr with 1 [micro]M BPDE.
In Figure 2C and D, the genes are rank ordered, not by dye-bias strength measured with the split controls as in Figure 2A and B but rather by the mean ratio values of the eight determinations from the two dye-swap experimental arrays, comparing a treated with a control sample (in this case RNA from TK6 cells treated with 1 [micro]M BPDE compared with the control sample, both from the 4-hr time point).
III] enhanced the formation of BPDE-DNA adducts by increasing the cellular uptake of BPDE.
In the present study BPDE was used to generate BPDE-DNA adducts.
To avoid hydrolysis of the epoxide, we always prepared a stock solution of BPDE fresh by dissolving BPDE in anhydrous tetrahydrofuran (THF) (> 99.
Cells were washed twice with phosphate-buffered saline (PBS) before the addition of BPDE in serum-free medium.
While 55% of BPDE-DNA adducts were removed within 24-hr after BPDE treatment in normal fibroblast CRL2522 cells, the differences in BPDE-DNA adduct levels in the GM04312C cells between different repair times up to 24 hr were not statistically significant (p > 0.
5 [micro]M BPDE, the BPDE-DNA adducts in the cellular DNA were measured by the CE-LIF-based immunoassay.