BPKIHSB.P. Koirala Institute of Health Sciences (Dharan, Nepal)
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Persons provided written consent before enrollment and providing blood samples, per the human experimentation guidelines approved by BPKIHS and the corresponding body at the Institute of Tropical Medicine, Antwerp.
All tubes were immediately stored in a chilled ice box and transferred on the same day to the laboratory at BPKIHS, where 180 [micro]L of each sample was transferred to a tube containing 180 [micro]L of AS1 buffer (catalog no.
56301; QIAGEN) was used to extract DNA at BPKIHS, following manufacturer's instructions.