Pedigree information was recorded for all cultivars cited as being BPMV, SMV, TRSV, or TSV-resistant.
Plants were inoculated with BPMV, SMV, TRSV, and TSV 7 to 10 d after planting at growth stage V1 (Fehr and Caviness, 1977).
Three weeks after inoculation, trifoliolate leaf samples were tested for the presence of BPMV, SMV, TRSV, and TSV by double antibody sandwich ELISA (Clark and Adams, 1977) using Agdia antibodies and protocol (Agdia, Inc.
Evaluation of Ancestral Lines for Resistance to BPMV, SMV, TRSV, and TSV
All ancestral lines tested were susceptible to the strain of BPMV used in this research (Table 1).
None of the cultivars were reported to be resistant to BPMV, TRSV or TSV, except the cultivar 'Semmes', which was reported to have a mild symptom reaction to BPMV (Ross, 1986).