Reextraction and testing of the original tick homogenates using both primer/probe sets NP1 and PB1 confirmed BRBV RNA in all 3 pools.
Analyses revealed 6 RNA segments for strain MO-2013-1246, as previously reported for the BRBV human strain (4).
The human BRBV strain from Kansas and tick pool MO-2013-1246 comprising male adult A.
Tick transmission of BRBV also is consistent with our knowledge of the vector status and phylogenetic relationships within the genus Thogotovirus and related viruses in the family Orthomyxoviridae (Figure 2).
Dhori virus, the virus most closely related to BRBV (4), is an Old World virus known from Europe, North Africa, and western and central Asia.