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BrdU |
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BrdU uptake experiments are therefore an effective indicator of cellular replication and proliferation. To determine the percentage changes in the fractions of cells in various cell cycle compartments with time post-IR, we incubated cells with BrdU for 2 hr beginning 2, 6, 12, and 24 hr after 1. They exhibit strong mitotic activity, which can be visualized by incorporating the thymidine analog BrdU (5-bromo-2'-deoxyuridine). |
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