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Cell-cycle analysis with use of Hoechst 33258 and propidium iodide or with incorporation of bromodeoxyuridine is briefly described.
As in most recent NSC experiments, the chemical market bromodeoxyuridine (BrdU) was used to measure cell proliferation (see the sidebar "Measuring Cell Proliferation").
To quantify the rate o f vascular cell proliferation, CL were obtained from ewes that were injected with bromodeoxyuridine (BrdU; a thymidine analog) 1h before slaughter on days 4, 8, 12, and 15 of their respective estrous cycle.
The chemicals, such as bromodeoxyuridine, or BrdU, become part of newly formed DNA when dividing cells copy their genetic information.
To enable detection of the transplanted cells, the cell cultures were labeled with a tracking marker bromodeoxyuridine (BrdU) which incorporates into actively dividing cells, prior to transplantation, a process which had no apparent effect on the growth or engraftment of the cells.
Cell proliferation was measured by the bromodeoxyuridine (BrdU) incorporation assay as described previously (Liu et at.
Approximately 50 cells are labeled with tritiated thymidine or its analogue, bromodeoxyuridine, for each cell in mitosis.
Histological evaluation revealed that bromodeoxyuridine (BrdU)-labelled [CD34.
The utilization of bromodeoxyuridine in corporation into DNA for the analysis of cellular kinetics.
The undifferentiated progenitor cells incorporated bromodeoxyuridine (BrdU) and showed nestin immunoreactivity.