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CALUX |
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To address the influence of spatial and temporal factors and to examine the potential biologic activity of the gas phase of organic contaminants in ambient air, we subjected extracts of particulate- and gas-phase ambient air samples, collected from both urban and rural locations in two seasons (winter and summer) over 2 years, to estrogen receptor (ER) chemically activated luciferase expression (CALUX) and aryl hydrocarbon receptor (AHR) CALUX cell bioassays. CALUX and high resolution GC/MS analysis of dioxin-like compounds in chlorophenoxy pesticide formulations. The cleaned extracts were dissolved in dimethyl sulfoxide (DMSO) for CALUX measurements in 96-well plates using H4IIE. |
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