In vitro secretion of cytokines by CBMCs after mitogenic stimulation was lower in the subsistence fishing group than in the reference group (p < 0.
CBMCs were incubated in triplicate at a concentration of 2 x [10.
Levels of cytokines spontaneously released by CBMCs in vitro were similar in both groups (Figure 2).
Levels of IL-10 secreted by PHA-stimulated CBMCs did nor exceed those spontaneously released for seven neonates from the subsistence fishing group and four neonates from the reference group (16% vs.
In this study we investigated whether or not the in vitro activation of CBMCs was altered in neonates from a subsistence fishing group who received an unusually high transplacental exposure to food-chain contaminants such as OCs and methyl mercury.
Cultured CBMCs from most neonates in our study spontaneously secreted detectable amounts of TNF-[alpha] but none exhibited signs of infection.