En el continente europeo se investiga principalmente TBEV, mientras que en Asia y Africa, las investigaciones estan encaminadas a la busqueda y control de CCHFV
, el cual es considerado uno de los virus mas letales entre los VTG.
can be isolated in a variety of cell lines including SW-13, Vero, LLC-MK2 and BHK-21 cells.
is mainly seen in the Middle East and Asia and parts of Europe including southern portions of the former Union of Soviet Socialist Republics (USSR).
It has been shown that livestock trade or movements of livestock or wild animals infested with infected ticks might be a culprit in dissemination of infected ticks, and hence spread of CCHFV
24] performed with cell cultures, nucleocapsid protein and viral RNA levels of CCHFV
have been found significantly decreased in cells with diminished cholesterol via methyl-[beta]-cyclodextrin administration.
The important members of the genus Nairovirus include CCHFV
, NSDV, in addition to GANV, HAZV and Dugbe virus (DUGV).
Kirim Kongo kanamali atesine neden olan CCHFV
kuresel yapidadir ve yaklasik olarak 90-100 nm capindadir, zarfli yapiya sahip olan virusun genetik yuku uc kisimdan olusan tek zincirli ve negatif polariteli RNA'dir (4).
has a negative-sense and tripartite RNA genome (small [S], medium [M], and large [L] segments) with high genetic diversity.
genome has been isolated from at least 31 different tick species in Ixodidae (hard ticks) and Argasidae (soft ticks) (11).
We attempted to isolate CCHFV
by inoculating infant CD-1 mice with 20 [micro]L of serum or blood collected from the patient on illness day 5.
Recent surveys have shown circulation of the virus in livestock existing in the province (16,20), but no further data on the genetic variability of the CCHFV
strain from the Hamadan province have been available.
Conventional IgM/IgG ELISAs were used for LASV (Corgenix, Broomfield, CO, USA) and CCHFV
(Vector-Best, Novosibirsk, Russia), and reagents for the EBOV IgM/IgG ELISA (infected/uninfected cell lysates) were prepared at the Rocky Mountain Laboratories (Hamilton, MT, USA) and validated with serum from experimentally infected monkeys.