CCOV

AcronymDefinition
CCoVCanine Coronavirus
CCOVColorado Conference on Volunteerism (Fort Collins, CO)
CCOVChrist Chapel of the Valley (California)
CCOVColombian Community of Victoria (Australia)
References in periodicals archive ?
Del analisis de factores de componentes principales del ccov se ratifica el impacto que a priori pueden tener los estudios de clima organizacional, en principio porque el exito en la gestion del clima organizacional esta precisamente en el nivel directivo y se expresa a traves del apoyo sistematico a los subordinados; por una parte, coincidiendo con los estudios de la motivacion hospitalaria realizados en el contexto chileno (15), porque los personales del sector de la salud valoran a sus jefaturas cuando se les respetan las opiniones y, por otra, cuando desde la gestion directiva superior se abren espacios de desarrollo profesional, se ejecutan buenas practicas y los funcionarios son reconocidos en la comunidad laboral.
A modo de reflexion, los diez factores determinados y los 44 reactivos definidos para medir el clima morganizacional en dos instituciones hospitalarias de alta complejidad, muestran la validez de las variables humanas, de relacionamiento, de confianza y lealtad que dan forma al constructo, de manera que seria necesario y recomendable, a nivel institucional del sector de la salud, realizar investigaciones de similar envergadura y complejidad tecnica en distintas reparticiones del sector de la salud, eventualmente extendiendo la aplicacion del ccov al sector de la atencion primaria de salud aps; ademas, de ser factible, su aplicacion a entidades hospitalarias de otras regiones de Chile.
Primer 20179 (sense, 5'-GGCTCTATCACATAACTCAGTCCTAG-3') binds a conserved region at the 3' end of ORF1b and was recruited from a previous study (13), whereas antisense primers INS-R-dg (5'-GCTGTAACATAKTCRTCATTCCAC-3') and 174-268 (5'-CAACATGTAACCTTTGTCTGTGATC TGC-3') target regions at the 5' end of the spike protein gene of feline CoV-II (FCoV-II)/classical CCoV-II and TGEV/TGEV-like CCoV, respectively.
Primers S5 (5'-TGCATTTGTGTCTCAGACTT-3') and S6 (5'-CCAAGGCCATTTTACATAAG-3') were used in the RT-PCR, performed according to the protocol described for CCoV subtyping.
To rule out the presence of true TGEV strains in the dog feces that were positive by CCoV-IIb-specific assay, we used an RT-PCR that had been proven to discriminate between TGEV and CCoV on the basis of amplicon size (19).
The geographic distribution of the CCoV types and subtypes is reported in Table 1.
After RT-PCR with primer pair N3SN/R3AS, CCoV and FCoV reference strains yielded an amplicon >1,000 bp, with the exception of TGEV-like CCoV 341/05, which gave a 929-bp product as a consequence of a 154-nt deletion in ORF7b (14).
All 20 samples that were positive according to the CCoV-IIb-specific assay and negative according to the CCoV-I TaqMan assay were confirmed to contain true CCoV strains because they yielded RT-PCR products considerably larger than the 367-bp amplicon obtained from TGEV.
8 kb) of the pantropic CCoV strain was determined by RT-PCR amplification and sequencing of overlapping fragments.
CCoV strain CB/05 showed intact structural and nonstructural proteins, with an S protein closely related to that of other type II CCoVs.
Accordingly, the appearance of pathogenic CCoV variants should always be regarded as a potential threat to domestic dogs and considered when unexplainable fatal disease outbreaks occur in puppies.
Epidemiologic studies are required to determine whether the pantropic CCoV strain is a new coronavirus variant emerging in canine populations or a widespread infectious agent of dogs that usually goes undetected.