CDNB

AcronymDefinition
CDNBConcise Dictionary of National Biography (Oxford University Press; UK)
CDNBCDR Discrepancy Notice Board (NASA)
References in periodicals archive ?
Glutathione s-transferase activity per mg weight and its specific activity were examined using CDNB as GST substrate for different life stages of different insecticideresistant strains and susceptible control.
Abbreviations: CDNB, 1-chloro-2,4-dinitrobenzene; CMNA, cyano(6-methoxy-2-naphthyl)methyl actetate; CMNPC, cyano(6-methoxy-naphthalen-2-yl)muthyl trans-[3-phenylaxiran-2-yl) methyl] carbonate; EROD, ethoxyresurufin; Octanoyl-MP, N-(6-methoxypyridin-3-yl) octanamide.
Although the presence of activity towards CDNB or other substrates is suggestive of the presence of enzymes from certain GST classes, it can by no means be regarded as proof of the presence of that specific class of GSTs.
For the CDNB endpoint method, only acidified clear supernatants were used: sample aliquots (0.
The GST activity was expressed in amount of conjugate constituted by GSH and CDNB catalyzed by GST per unit time per mg of enzyme (mol min-1 mg-1 protein).
After centrifugation, the CDNB conjugate was measured spectrophotometrically at 340 [eta]m.
Kinetic, electrophoretic, and immunoblotting data confirmed the presence of GST-[pi] in the purified enzyme preparations; specific activity was measured using CDNB as substrate.
Change in absorbance per min was converted into micromoles of CDNB conjugated per min per mg of protein using the extinction coefficient (9.
It is based on the conjugation reaction between GST and a substrate, CDNB (1-chloro 2, 4 dinitrobenzene) in the presence of a cofactor: glutathione (GSH).
Working solution (phosphate buffer 100mM, GSH 1mM, EDTA 60mM), CDNB (10mM) and sample were placed in a cuvette and mixed.