CDNBConcise Dictionary of National Biography (Oxford University Press; UK)
CDNBCDR Discrepancy Notice Board (NASA)
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max]) of the purified GSTs from the three developmental stages were determined for GSH or CDNB by recording the activity towards a range of GSH (2 to 24 mM) or CDNB (0.
Glutathione s-transferase activity per mg weight and its specific activity were examined using CDNB as GST substrate for different life stages of different insecticideresistant strains and susceptible control.
Abbreviations: CDNB, 1-chloro-2,4-dinitrobenzene; CMNA, cyano(6-methoxy-2-naphthyl)methyl actetate; CMNPC, cyano(6-methoxy-naphthalen-2-yl)muthyl trans-[3-phenylaxiran-2-yl) methyl] carbonate; EROD, ethoxyresurufin; Octanoyl-MP, N-(6-methoxypyridin-3-yl) octanamide.
Although the presence of activity towards CDNB or other substrates is suggestive of the presence of enzymes from certain GST classes, it can by no means be regarded as proof of the presence of that specific class of GSTs.
For the CDNB endpoint method, only acidified clear supernatants were used: sample aliquots (0.
Change in absorbance per min was converted into micromoles of CDNB conjugated per min per mg of protein using the extinction coefficient (9.
1% triton X-100) after adding 20 [micro]l reduced glutathione, the reaction was initiated by adding 10 [micro]l CDNB, and then the plate was shake for few seconds and the optical density was measured at 340 nm once every min to obtained at least 5 time points and its activity was measured as (nmol/min/ml).
After centrifugation, the CDNB conjugate was measured spectrophotometrically at 340 [eta]m.
Kinetic, electrophoretic, and immunoblotting data confirmed the presence of GST-[pi] in the purified enzyme preparations; specific activity was measured using CDNB as substrate.
The reaction solution, which contained 100 mL each of the supernatant, CDNB in ethanol (0.