The chromatographic conditions for glycyrrhizin were column: a stainless steel column packed with octadecylsilanized silica gel for liquid chromatography, mobile phase: a mixture of H2O, CH3CN and CH3COOH
, column temperature: a constant temperature of about 40 C, flow rate: 1.
1 and 1M) like HNO3, HCl, EDTA, CaCl2, KCl, NaHCO3 and CH3COOH
was examined to recover nickel from biosorbed bacterial cells of the selected strain at 30[degrees]C and 150 rpm.
After zinc and nickel ions sorption, the biomass was filtered, washed three times with distilled water to remove residual nickel ions on the surface, and kept in contact with the 100 ml desorbent solution: HNO3, HCl, EDTA, CH3COOH
and distilled water.