The fact that electrophoresis shows 100% CK-MM
, however, suggests that skeletal muscle is the origin of increased CK activity.
To identify the CK-M protein expression, 15 [micro]g of homogenates from cardiac and skeletal muscles of the patient and from the control, along with purified human CK-MM (BiosPacific) were immunoblotted with polyclonal goat anti-CK-M (BiosPacific).
Serum CK-MM activity was substantially reduced, and no serum CK-MB activity was detected in the patient sample.
The hybrid CK-MB, prepared by reassociation of the respective M and B subunits of denatured CK-MM and CK-BB, was obtained from Aalto Scientific.
11] Nonstandard abbreviations: CK, creatine kinase; CK-MB, CK-MM, and CK-BB, isoenzymes MB, MM, and BB of CK, respectively; AMI, acute myocardial infarction; CK-M and CK-B, M and B subunits of CK, respectively; rCK-MB2, recombinant CK-MB2 (tissue isoform); SDS-PAGE, sodium dodecyl sulfate-polyacrylamide gel electrophoresis; and rCK-MB1, recombinant CK-MB1 (serum isoform, missing the C-terminal lysine on the M subunit).
Maturing chick embryo skeletal muscle shows progression of CK isoenzyme content from CK-BB to CK-MB to CK-MM (6).
CK isoenzymes (RI), LD isoenzymes (RI), U/L U/L CK-MM 130 (0-170) [LD.
predominants in striated muscle, both skeletal and myocardial.
Macro CK type 1 (CK-BB bound to IgG) migrates between CK-MM
and -MB, whereas type 2 (polymeric mitochondrial CK) migrates cathodic to CK-MM
and can be readily differentiated from CK-MB by electrophoresis .
CK-MB (reference value, [less than or equal to]5 [micro]g/L) was measured by a mass immunoassay based on a monoclonal antibody that recognizes CK-MB, but neither CK-MM
nor CK-BB, on the Stratus II Analyzer (Dade International, Miami, FL) .