Using a modified ophthalmoscope to monitor the retinas of mice, researchers observed that moderate doses of CNTF
CNTF acts as a survival factor for a variety of neuronal cell types, including sensory, motor, cerebral, hippocampal and hypothalamic magnocellular neurons (MCN).
Quantitative analysis demonstrates that there is a significant decrease in CNTF protein expression in response to hypernatremia; however, it is still uncertain what leads to this decrease in protein expression and future studies will be performed to help answer this question.
In summary, CNTF expression has previously been demonstrated to change only in response to injury; yet, our research has demonstrated that CNTF expression can be changed in an activity-dependent fashion independent of cellular injury.
5A) and CNTF
, EGF, IGFBP4, and BMP6 (other responses, Fig.
In contrast, transplantation of the CNTF
astrocytes, or of the stem cells themselves, failed to provide these benefits.
Sieving presented the results last November at the American Academy of Ophthalmology, and the results confirmed that CNTF can be safely delivered into the vitreous of patients with RP.
The implant is designed to continuously release CNTF directly in the eye to the diseased retinal tissue for sustained periods of time.
Early clinical trials with classical neurotrophic growth factor proteins such as NGF, BDNF, CNTF
have failed for various reasons including safety, efficacy and convenience of administration as well as right definition of the clinical indication.
Based on the increase in retinal thickness observed in this study it appears that CNTF may be exhibiting a biological effect on retinal photoreceptors as has been observed previously in animal studies," said Dr.
CNTF is delivered directly to the back of the eye in a controlled, continuous basis by means of the Company's proprietary Encapsulated Cell Technology (ECT) platform, thereby bypassing the blood-retinal barrier and overcoming a major obstacle in the treatment of retinal disease.
The observed structural change is consistent with preclinical studies of NT-501 in which CNTF was shown to increase the thickness of the retina and the outer nuclear layer of photoreceptors responsible for vision.