Thus, 14 sequences were analyzed for COI, r16S, and COI + COIII, and 19 sequences for COIII (Appendix A).
All three mitochondrial gene regions analyzed in the Octopus species showed bias against G + C content, which prompted for the analysis of codon usage in COI and COIII regions.
Other nonsynonymous substitutions detected in COIII sequences were two transitions at positions 427 and 502, and a transversion at position 429.
hubbsorum whereas two haplotypes for COI and r16S, and 4 for COIII were resolved for O.
The lowest divergences between taxa for COI, COIII, and r16S were 8.
In the Bayesian COIII topology, the clade conformed by O.
Only a transversion in the COIII sequence between Octopus hubbsorum and Octopus mimus was observed, suggesting they are still in a divergent process or they diverged recently.
9% (Keskin & Huseyin 2011); in South Africa, the intraspecific divergence, using COIII, for Octopus vulgaris ranged from 0.
The greatest distance value for the genes COIII and r16S between O.
Nevertheless, the resolved topologies using COIII and r16S gene regions showed a polytomy of O.
Topologies of Octopus hubbsorum-Octopus mimus specimens constructed using Bayesian analysis based on 3 genes: COI, COIII, and r16S.
Topologies of Octopus hubbsorum-Octopus mimus specimens constructed using parsimony based on 3 genes: COI, COIII, and r16S.