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For PCR-RFLP analysis of the COWP gene, PCR was carried out by using the forward primer 5'-GTAGATAATGGAAGAGATTGTG-3' and reverse primer 5'-GGACTGAAATACAGGCATTAT CTTG-3' to produce an amplicon of [approximately equal to] 550 bp. Valencia, CA, USA), and 1 [micro]L DNA was used in nested PCR analyses of the DHFR and COWP genes. parvum genotype in human fecal samples was first determined by a COWP gene-based PCR-RFLP tool (10). |
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