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In this test, animals were individually placed on a hot-plate (May AHP 0603 Analgesic Hot-plate Commat, Turkey) with the temperature adjusted to 54 [+ or -] 3[degrees]C.
Adaptation Training: For the rats to have adaptation they were made to have exercise on treadmill for 10-15 min, at 20 m/min during per week (MAY TME 9805 treadmill exerciser, Commat Ltd.
Animals were exhausted on a motor-driven treadmill (MAY-TME 9805, Commat, Ankara, Turkey) for 5 days.