Cos7 (African green monkey epithelial kidney cells) and HeLa (cervical cancer) cell lines were maintained in DMEM complete media (L-glutamine supplemented with 10% heat-inactivated fetal bovine serum, 5% penicillin/streptomycin and 5% non-essential amino acids).
5-diphenyltetrazolium bromide (MTT) cell viability assay (Mosmann, 1983) was used to determine the cytotoxicity of monoterpenes and saponins in the cancer cell lines HeLa and Cos7.
Cos7 and HeLa cells were treated with a dilution series of the saponins aescin, digitonin, glycyrrhizic acid and Quillaja saponin (containing 20-35% quillajic acid) (Fig.
In a consecutive experiment, Cos7 cells were treated with a dilution series of the saponins aescin, digitonin, glycyrrhizic acid and Quillaja saponin in combination with a-pinene and thymol.
In a second set of experiments the cell lines HeLa and Cos7 were treated with saponins in combination with monterpenes.
For radiolabeling of COS7, cells were seeded in 6-well tissue culture dishes in 0.
COS7 cells were seeded at 80,000 cells per well in 24-well tissue culture plates in 1 mL DMEM with 10% FBS.
2005), we assessed readthrough of the UGA codon [with appropriate selenocysteine insertion sequence (SECIS) element in the sense configuration] when COS7 cells were treated with arsenite.
To confirm that the impact of arsenite would be similar to that seen in other cell types (HaCat, HeLa, NIH3T3), we treated COS7 with 2 or 6 [micro]M arsenite in the presence of radioisotope selenium (Figure 8B).