For the treatment with CuCl2, a solution of 2 mM CuCl2 was sprayed three time, once on 25 days old plants leaves.
Control plant (without any treatment) and CuCl2 control (plants sprayed with CuCl2 just before harvesting) were also raised parallel with treatment plant, harvested, processed in the same age as treatment one.
The inducibility of the synthesis of antifungal compounds by abiotic and biotic stresses was studied by exposing the plants to UV-light, treating them with CuCl2 and inoculating them with pathogens M.
NaBH4 in the presence of NiCl2 [10,11], CoCl2 [12,13], CuCl2
, ZrCl4 , SnCl2 , BiCl3 , SbF3 , CuSO4 , Raney nickel , Ni2B , nickel complexes of o-aminothiophenol Schiff base  and charcoal  are some of the combination systems which have been reported for reduction of nitro compounds to their corresponding amines.
ReagentsAnalaR grade chemicals: 4- Florobenzaldehyde, 1,4-naphthquinone, ethylenediamine, m-creosol, n-hexane, tetrahydro- furan (THF), dimethylformamide (DMF), dimethylacetamide (DMAc), dimethylsulphoxide (DMSO), NiCl2 and CuCl2
were obtained from Fluka, Switerzerland).