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The results from the evaluation of the 17 target transcripts representing the product of genes whose transcription is regulated by estrogens, as well as two genes not regulated by estrogens (internal controls: vascular [alpha]-actin and cyclophilin B), from the uterus/ovaries of the immature rats exposed to 10 [micro]g EE/kg/day (high dose) are shown in Table 3. The human L13 gene and the human cyclophilin gene (16) were amplified under identical reaction conditions as the SARS-CoV--specific assays on the ABI platforms and the LightCycler, respectively, to act as amplification controls. Unlike many other proteins, which twist or bend to make a tight fit with their partner molecules, cyclophilin maintains its shape when it binds to cyclosporin, adds Malcolm D. |
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